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New DNA based assay rapidly identifies E. coli

Started by Andrew Yates · 9 months ago

My first paper just got published in this month’s issue of the Journal of Clinical Microbiology. We, primarily researchers at Children’s Hospital of Pittsburgh, developed an assay using a technique called Loop Mediated Isothermal Amplification (LAMP) to identify E. co ... Continue reading »

4 comments

  • Andrew Yates

    11 months ago

    Congratulations!
    reply
  • Nikhil Chopra

    11 months ago

    Great. How much do you think would you test cost in a developing country when commercialized ?
    reply
  • Josh Hill

    11 months ago

    Reagent costs themselves should be more than a few dollars. The hard part is going to be keeping the reagents cold/frozen in villages without electricity. I read about an invention a few months ago about a device that you heated and it cooled things for several hours. But many other techniques exist, such as http://www.newmediaexplorer.org/chris/2004/04/1...

    We did an experiment with those chemical hand warmers you guy at Wal-mart or any other store, and stuck a tube in them. It was the perfect temperature for the reaction, and an hour later we got a positive result.

    So in short, this should be very cheap and practical in developing countries.
    reply
  • John C

    11 months ago

    So...first author is the one who does most of the work and the write-up; at least that is the way at Stanford. The last author is usually the Principal Investigator (PI), also known in graduate school parliance as 'the slave master' or, as in Disney's film "Fantasia", 'the Sorcerer'. Good!
    reply

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